Which movement sought to end the prohibition of alcohol in the U.S.?

The correct choice is the Temperance Movement. This movement emerged in the 19th century and was characterized by a strong advocacy for the reduction or elimination of alcohol consumption. Its advocates believed that alcohol was the root of many societal problems, including crime and poverty. The movement gained momentum and was instrumental in leading to the establishment of Prohibition in the 1920s through the 18th Amendment.

While the Women's Suffrage Movement focused on securing women's right to vote, and the Civil Rights Movement aimed at achieving equal rights and ending segregation, these movements did not have alcohol prohibition as their primary goal. The Labor Movement worked towards improving working conditions and workers' rights, which also did not include the specific focus on alcohol prohibition. Thus, the Temperance Movement is distinctly linked to the efforts to end the prohibition of alcohol, making it the correct answer.